Effect of Ag nanoparticles on viability of MCF-7 and Vero cell lines and gene expression of apoptotic genes

Abstract Background The newly emerged technology, nanotechnology, represents a promising solution for many medical and industrial problems. Random targeting, resistance, side effects are the main disadvantages of available cancer chemotherapy which critical aspects needed to be managed. So aim study was suggest nanoparticles as an alternative therapy therapies through detecting cytotoxic effect Ag against normal cell lines how they affect apoptotic function genes involved. Results NPs exhibited killing rate 40% in MCF-7 cells (the model) at concentration 100 ?g/ml with almost no on Vero model). Concerning phenotypic changes that were analyzed by Acridine orange eosin hematoxylin, caused apoptosis Vacuole degeneration well formation emergence Necrotic cells, whereas line Vero, change appears its phenotype. Treating upregulated P53 P21 gene expression but their downregulated cells. PTEN augmented both compared control. Conclusions AgNPs displayed selective cytotoxicity induced might suggested potential since increase (up 250-fold more control) due treatment augments tumor suppressor PTEN.